Signaling pathways used by EGF to stimulate conjunctival goblet cell secretion
The purpose of this study was to identify the signaling pathways that epidermal growth factor
(EGF) uses to stimulate mucin secretion from cultured rat conjunctival goblet cells and to
compare the pathways used by EGF with those used by the known secretagogue
muscarinic, cholinergic agonists. To this end, goblet cells from rat conjunctiva were grown in
culture using RPMI media. For immunofluorescence experiments, antibodies against EGF
receptor (EGFR) and ERK 2 as well as muscarinic receptors (M1AchR, M2AchR, and …
(EGF) uses to stimulate mucin secretion from cultured rat conjunctival goblet cells and to
compare the pathways used by EGF with those used by the known secretagogue
muscarinic, cholinergic agonists. To this end, goblet cells from rat conjunctiva were grown in
culture using RPMI media. For immunofluorescence experiments, antibodies against EGF
receptor (EGFR) and ERK 2 as well as muscarinic receptors (M1AchR, M2AchR, and …
